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Y. Zhang, I. Schmack, D.G. Dawson, H.E. Grossniklaus, A.H. Conrad, Y. Kariya, K. Suzuki, H.F. Edelhauser, G.W. Conrad; Analysis of Oligosaccharides Derived From Keratan Sulfate and Chondroitin/Dermatan Sulfate Disaccharides in Post–Mortem Human LASIK Corneas Using Laser Capture Microscopy and Mass Spectrometry . Invest. Ophthalmol. Vis. Sci. 2006;47(13):3002.
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© ARVO (1962-2015); The Authors (2016-present)
To analyze the amounts and distributions of non–sulfated and sulfated keratan sulfate (KS) and chondroitin/dermatan sulfate (CS/DS) disaccharides in the interface wound of human post–mortem LASIK corneas in comparison to human non–wounded normal corneas.
Corneal stromal tissue samples from central and paracentral hypocellular primitive stromal interface wounds of human post–LASIK corneas and from similar regions of normal corneas were collected by laser capture microdissection (LCM), and subsequently digested with specific glycosidase enzymes. Digests were directly analyzed by electrospray ionization tandem mass spectrometry (ESI–MS/MS).
Concentrations of both monosulfated GlcNAc(6S)–ß–1,3–Gal (MSD2) and disulfated Gal (6S)–ß–1,4–GlcNAc(6S) (DSD) KS disaccharides in LASIK interface wounds are significantly lower compared to normal corneal stroma. No significant difference is found in the concentration of non–sulfated (NSD) KS disaccharides in LASIK interface wounds compared to normal corneas. On the other hand, concentration of ΔUA–ß–1,3–GalNAc(6S) (Δdi–6S) CS/DS disaccharides in the LASIK interface wound is significantly higher than normal corneal stroma, whereas concentrations of ΔUA–ß–1,3–GalNAc(4S) (Δdi–4S) and non–sulfated Δdi–0S CS/DS disaccharides are not significantly different from normal.corneas.
The profiles of KS and CS/DS disaccharides in LASIK interface wounds are significantly different from normal cornea stromal tissue, as revealed by LCM and ESI–MS/MS.
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