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R.I. Martinez, S. Nekkalapudi, H.M. El–Hodiri; Expression of the Retinal Homeobox (RX) Gene Is Up–Regulated During Xenopus Laevis Retinal Regeneration . Invest. Ophthalmol. Vis. Sci. 2006;47(13):3120.
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The retina in Xenopus laevis tadpoles can regenerate following partial retinal removal. Rx is essential for normal eye development, suggesting it may also play a role during retinal regeneration. During normal development, Rx expression begins in the presumptive eye fields at the onset of neurulation and then becomes restricted to the photoreceptor layer and to the progenitor cells in the ciliary marginal zone (CMZ) of the maturing retina. Here we investigate the expression of Rx in the regenerating Xenopus laevis embryonic retina.
A nasal–dorsal segment was partially removed from the retina of stage 44 tadpoles. The tadpoles were then fixed daily after surgery for 7 consecutive days. Rx expression was examined by in situ hybridization of coronal eye sections. For comparison, Neuro D and Pax 6 transcription levels were also assessed.
We found that Rx transcription is increased in the retina of regenerating eyes as compared to control eyes. Interestingly, the increased expression of Rx was not limited to the site of injury, but observed in specific layers of the retina throughout the regenerating eye. Neuro D and Pax 6 expression was also increased, but unlike Rx, the increased transcription was restricted to the injury site.
Upon injury to the retina, Rx transcription appears to increase. This increase in Rx transcription suggests that Rx may play an important role in retinal regeneration.
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