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H.B. Ahn, J.M. Woo, Y.H. Kwon, W.C. Park, K.W. Yoo, S.H. Rho; The Efficacy of Amniotic Membrane Graft With Adhesiolysis in Experimentally Induced Lid Adhesion Rabbit . Invest. Ophthalmol. Vis. Sci. 2006;47(13):3772.
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We investigated the anti–inflammatory and anti–fibrotic effect of amniotic membrane(AM) on secondary blepharoplasty with adhesiolysis in the rabbit model.
After intramuscular anesthesia on 8 albino rabbits, primary blepharoplasty was done. After 2 weeks, new skin incision was made, adhesiolysis was done on both eyelids. We categorized two groups, adhesiolysis alone in left eyelids(control), adhesiolysis with AM graft in right eyelids(AM graft). In AM graft group, the 15 x 4mm sized amniotic membrane was designed and applied between tarsal plate, orbicularis muscle and subcutaneous tissue with 8–0 vicryl suture(4 points). The skin was sutured with 6–0 nylon. The left eyelids was served as a control, adhesiolysis alone. After operation, the rabbits were sacrificed in 2, 4 and 8 weeks, and evaluated the change of lid shape, inflammation and fibrosis. The degree of inflammatory cell infiltration was evaluated on H & E stain and Masson–trichrome stain was performed to evaluate the degree of fibrosis.
On eyelid examination, directly after the operation, the lid retraction detected in both groups, but after 1 week, the eyelid closes completely. At 8 weeks, the control group showed more irregular lid margin and surface than AM graft group. In microscopic findings, at 2 weeks, the AM graft group showed more inflammatory cell infiltration than control group. At 4 weeks, amniotic membranes were absorbed and inflammatory cell infiltration decreased. But AM graft group showed more inflammation than control group. At 8 weeks, two groups had little inflammation, but the AM graft group showed less fibrosis than control group on Masson–trichrome stain.
These results indicate that amniotic membrane graft reduces the fibrosis on blepharoplasty. From these findings, we suggest that the use of amniotic membrane graft After adhesiolysis and blepharoplasty is an effective method for prevention of postoperative adhesion.
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