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H.–K. Kuo, P.–C. Wu, Y.–H. Chen, Y.–C. Wu; A Comparative Study of Effects of Topoisomerase Inhibitors on Retinal Pigment Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2006;47(13):3826.
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© ARVO (1962-2015); The Authors (2016-present)
The inhibition of RPE proliferation by chemotherapeutic agents remains a primary target in the treatment of PVR. In this study, we investigate the effect of some commercially available topoisomerase inhibitors on the RPE cells.
Study medications included topotecan, etoposide, doxorubicin, and daunorubicin at various concentrations (100, 50, 30, 10, 1, 0.1, 0.01 ug/ml). Rabbit RPE cells were used. After 48 and 96 hours, MTT assay and cell counting were used to determine cell survival and proliferation rate.
Four drugs all inhibited proliferation of RPE cells in a dose–dependent manner in vitro. The actual numbers of cells correlated well with the results of the MTT test. The IC50 is defined as the concentration of a drug that produces a 50% reduction in cell number. The IC50 of topotecan, etoposide, doxorubicin, and daunorubicin was 0.1–1, 1–10, <0.1, and <0.1 ug/ml respectively at 48hours. The slope of dose–response curve was slowly declined for topotecan and etoposide, but very sharp for doxorubicin and daunorubicin.
Topotecan and etoposide have both effective and safe property as RPE cells inhibitors.
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