May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
The Effects of Glucose Concentrations on Reactive Oxygen Products in Human Lens Epithelial Cells
Author Affiliations & Notes
  • J. Koh
    Ophthalmology, Chosun University Hospital, Gwanju, Republic of Korea
  • Y. Yang
    Ophthalmology, Seoul Veterans Hospital, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  J. Koh, None; Y. Yang, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 4111. doi:
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      J. Koh, Y. Yang; The Effects of Glucose Concentrations on Reactive Oxygen Products in Human Lens Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4111.

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Abstract

Purpose: : To investigate the effects of glucose concentrations on formation of reactive oxygen products and cellular injury in human lens epithelial(HLE) cells.

Methods: : Human lens epithelial(HLE) cells were cultured with high glucose(200mg/100ml, 300mg/100ml, 400mg/100ml) and normal glucose(100mg/100ml) contained MEM. The amount of reactive oxygen products formation were measured by using dihydroethidium(DHE). Paraquat–induced cytotoxicity was determined by 3–[4,5–dimethylthiazol–2–yl]–2,5 –diphenyl tetrazolium bromide (MTT) method.

Results: : Reactive oxygen products of human lens epithelial cells were increased 110%, 250%. 630% in high glucose(200mg/100ml, 300mg/100ml, 400mg/100ml) media compared to normal glucose(100mg/100ml) media. The paraquat–induced cells toxicity was increased by high glucose.

Conclusions: : High glucose increased formation of reactive oxygen products in human lens epithelial cells. These results suggest that high glucose can induce cataract formation in diabetic patient.

Keywords: oxidation/oxidative or free radical damage • diabetes • cataract 
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