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K. Yamada, E. Sakurai, S. Yamasaki, M. Itaya, Y. Ogura; Atorvastatin Inhibits Laser–Induced Experimental Choroidal Neovascularization Through Downregulation of Vascular Endothelial Growth Factor . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4154.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the effect of atorvastatin, HMG CoA reductase inhibitor, on experimental choroidal neovascularization (CNV) induced by laser photocagulation in mice.
CNV was experimentally induced by the laser photocoagulation in normal wild–type (C57Bl/6J) mice. The mice orally received atorvastatin 10 mg/kg/day (AS10 group, n=10) or 20 mg/kg/day (AS20 group, n=10) for 3 days prior to laser application. Development of CNV was studied on the basis of dimension measurements obtained by confocal microscopy 1 week after laser application. VEGF protein levels from RPE/choroid lysates were measured by ELISA at 3 days after photocoagulation. Macrophage infiltration to RPE /choroid was quantitatively studied by flow cytemetry getting with F4/80+ cells.
Mean CNV area was significantly smaller in the AS10 (non–paired t test, 11249.30±2353.85µm2, p<0.001) and AS20 (13717.13±3357.36µm2, p=0.008) groups compared to control mice (26565.65±3180.67µm2). Mean VEGF levels were significantly reduced in AS10 (62.3±4.7%, p=0.001) and AS20 (52.9±4.5%, p=0.004) groups compared to the controls. The numbers of macrophage infiltrated into the RPE/choroid was also reduced in AS10 (64.2±4.5%, p=0.03) and AS20 (58.5±7.2%, p=0.01) groups.
Atorvastatin treatment effectively inhibited the laser–induced CNV in mice. It was associated with the downregulation of VEGF and the reduced macrophage infiltration in the choroids.
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