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M.V. Volin, R. Trachimowicz, N. Qamar, J. Vergin, B. Colander, J. Radliff, III; Expression of CXCL16 and Its Receptors CXCR6 in Rat Endotoxin–Induced Uveitis . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4531.
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Acute anterior uveitis is characterized by invasion of leukocytes into the anterior chamber of the eye. The CXC chemokine, CXCL16, is involved in recruiting leukocytes to sites of inflammation. CXCL16 functions by binding a specific receptor, CXCR6, on the surface of leukocytes resulting in their chemotaxis to the site of inflammation.
Immunohistochemistry was performed to determine the expression pattern of CXCL16 and its receptor CXCR6 throughout the course of uveitis in a rat endotoxin–induced uveitis model. In this model inflammation, as determined by slit lamp examination, peaks at 24 hours following injection of LPS and continues for several days. We examined 12–13 rats at the time of injection (0 hour), 6 hours, 18 hours, 24 hours, and 72 hours after injection. Immunostained rat eyes were microscopically analyzed by two blinded observers and staining was quantified on a 10 point scale with 1 indicating no staining and 10 indicating all cells staining.
CXCL16 was expressed by cells of the ciliary body and iris. In the ciliary body, CXCL16 expression significantly increased at 6 hours following LPS injection and stayed significantly elevated through the 18 hour time point (n=12, p<0.05). In the iris the expression of CXCL16 paralleled its expression in the ciliary body but did not reach statistical significance. CXCR6 was also expressed on cells in the iris and ciliary body. In the ciliary body, CXCR6 showed a biphasic expression, first being significantly increased at 6 hours and 18 hours following LPS injection before returning to baseline at 24 hours and rising again 72 hours after LPS injection (n=11, p<0.05). In the iris, CXCR6 expression paralleled its expression in the ciliary body but only reached significance at the 72 hour time point (n=8, p<0.05).
This data suggests a role for CXCL16 and its receptor, CXCR6, in the pathogenesis of acute anterior uveitis.
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