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B. Manickan, P. Jha, Q. Xu, J.–H. Sohn, P. Bora, N. Bora; Expression of C3a and C5a Receptors During Experimental Autoimmune Anterior Uveitis (EAAU) . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4534.
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© ARVO (1962-2015); The Authors (2016-present)
To study the expression of C3a receptor (C3aR) and C5a receptor (C5aR) during experimental autoimmune anterior uveitis (EAAU).
EAAU was induced by immunization with bovine melanin associated antigen (MAA). Clinical and histopathological examination was used to determine the onset and severity of the disease. Lewis rats were sacrificed at day 14, 17 and 20 post immunization. Eyes were harvested at these time points for Western blot and immunohistochemical analysis for C3aR and C5aR.
Immunohistochemical staining and Western blot analysis revealed increased expression of C3aR and C5aR in rat eyes with EAAU at day 14, 17 and 20. Weak expression of C3aR and C5aR was observed in the eye of naïve Lewis rats.
Our results demonstrate that the expression of C3aR and C5aR was up–regulated during the onset and peak of EAAU. These results suggest that binding of complement activation products – C3a and C5a to their receptors may play an important role in EAAU.
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