May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Expression of C3a and C5a Receptors During Experimental Autoimmune Anterior Uveitis (EAAU)
Author Affiliations & Notes
  • B. Manickan
    Ophthalmology, Jones Eye Institute/ UAMS, Little Rock, AR
    Ophthalmology, Kentucky Lions Eye Center/ University of Louisville, Louisville, KY
  • P. Jha
    Ophthalmology, Jones Eye Institute/ UAMS, Little Rock, AR
    Ophthalmology, Kentucky Lions Eye Center/ University of Louisville, Louisville, KY
  • Q. Xu
    Ophthalmology, Jones Eye Institute/ UAMS, Little Rock, AR
    Ophthalmology, Kentucky Lions Eye Center/ University of Louisville, Louisville, KY
  • J.–H. Sohn
    Ophthalmology, Kentucky Lions Eye Center/ University of Louisville, Louisville, KY
  • P. Bora
    Ophthalmology, Jones Eye Institute/ UAMS, Little Rock, AR
    Ophthalmology, Kentucky Lions Eye Center/ University of Louisville, Louisville, KY
  • N. Bora
    Ophthalmology, Jones Eye Institute/ UAMS, Little Rock, AR
    Ophthalmology, Kentucky Lions Eye Center/ University of Louisville, Louisville, KY
  • Footnotes
    Commercial Relationships  B. Manickan, None; P. Jha, None; Q. Xu, None; J. Sohn, None; P. Bora, None; N. Bora, None.
  • Footnotes
    Support  EY13335, EY014623 HIGHWIRE EXLINK_ID="47:5:4534:1" VALUE="EY014623" TYPEGUESS="GEN" /HIGHWIRE , EY016205 HIGHWIRE EXLINK_ID="47:5:4534:2" VALUE="EY016205" TYPEGUESS="GEN" /HIGHWIRE , R24 EY016636 HIGHWIRE EXLINK_ID="47:5:4534:3" VALUE="EY016636" TYPEGUESS="GEN" /HIGHWIRE , Commonwealth of Kentucky Research Challenge Trust Fund and Research to Prevent Blindness, Inc. NY.
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 4534. doi:
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    • Get Citation

      B. Manickan, P. Jha, Q. Xu, J.–H. Sohn, P. Bora, N. Bora; Expression of C3a and C5a Receptors During Experimental Autoimmune Anterior Uveitis (EAAU) . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4534.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To study the expression of C3a receptor (C3aR) and C5a receptor (C5aR) during experimental autoimmune anterior uveitis (EAAU).

Methods: : EAAU was induced by immunization with bovine melanin associated antigen (MAA). Clinical and histopathological examination was used to determine the onset and severity of the disease. Lewis rats were sacrificed at day 14, 17 and 20 post immunization. Eyes were harvested at these time points for Western blot and immunohistochemical analysis for C3aR and C5aR.

Results: : Immunohistochemical staining and Western blot analysis revealed increased expression of C3aR and C5aR in rat eyes with EAAU at day 14, 17 and 20. Weak expression of C3aR and C5aR was observed in the eye of naïve Lewis rats.

Conclusions: : Our results demonstrate that the expression of C3aR and C5aR was up–regulated during the onset and peak of EAAU. These results suggest that binding of complement activation products – C3a and C5a to their receptors may play an important role in EAAU.

Keywords: uveitis-clinical/animal model • autoimmune disease • inflammation 
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