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E. Fahl, M. Samardzija, N. Tanimoto, F. Tonagel, J. von Lintig, C. Grimm, M.W. Seeliger; Time Course of Retinal Light Damage in vivo Revealed by SLO Imaging and ERG . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4566.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the time course of light exposure–related damage on retinal structure and function in mice.
WT mice (mixed background of C57BL/6 and 129/Sv on the Rpe65–Leu450 variant) were exposed to bright light (15000 lux) for two hours. The time course of changes to retinal function and morphology was assessed between 1 and 70 days post exposure using flash electroretinography (ERG), scanning laser ophthalmoscopy (SLO), and histology. The study was performed in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research.
Retinal function was severely impaired already at day 1 after light exposure, and recovered only slightly over the observation period. In contrast, perceivable structural fundus changes, mainly indicating RPE/outer retinal pathology, developed gradually up to a maximum at about a month post exposure. Particles autofluorescent at 488 nm were present from day 5 on, and decreased slowly to about one third until day 70. Interestingly, there was also autofluorescence in the infrared range (795 nm), which developed much later and peaked at day 30 post exposure.
This study demonstrates the long–term effects following bright light exposure–induced apoptosis on morphology and function of the retina. The functional data indicate an almost complete, rapid loss of photoreceptors. The autofluorescent particles observed correspond presumably to phagosomes containing the lipid–rich remains of outer segment material. The changes in autofluorescence over time may thus reflect different stages of degradation of the debris.
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