May 2006
Volume 47, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2006
Retinal Transplantation Improves Host Cone Survival in the P23H Rat Model of Retinitis Pigmentosa.
Author Affiliations & Notes
  • Y. Yang
    Inserm U592, Hopital Saint Antoine, Université de Paris VI, Paris, France
    Cell and Molecule Physiopathology of Retina,
  • S. Mohand–Said
    Inserm U592, Hopital Saint Antoine, Université de Paris VI, Paris, France
    Cell and molecule physiopathology of retina,
    Ophtalmologie Service, Centre Hospitalier National d'Ophtalmologie des Quinze–Vingts, Paris, France
  • V. Fontaine
    Inserm U592, Hopital Saint Antoine, Université de Paris VI, Paris, France
    Cell and molecule physiopathology of retina,
  • M. Simonutti
    Inserm U592, Hopital Saint Antoine, Université de Paris VI, Paris, France
    Cell and molecule physiopathology of retina,
  • T. Léveillard
    Inserm U592, Hopital Saint Antoine, Université de Paris VI, Paris, France
    Cell and molecule physiopathology of retina,
  • J.–A. Sahel
    Inserm U592, Hopital Saint Antoine, Université de Paris VI, Paris, France
    Cell and molecule physiopathology of retina,
    Ophtalmologie Service, Centre Hospitalier National d'Ophtalmologie des Quinze–Vingts, Paris, France
  • Footnotes
    Commercial Relationships  Y. Yang, None; S. Mohand–Said, None; V. Fontaine, None; M. Simonutti, None; T. Léveillard, None; J. Sahel, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2006, Vol.47, 4803. doi:
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      Y. Yang, S. Mohand–Said, V. Fontaine, M. Simonutti, T. Léveillard, J.–A. Sahel; Retinal Transplantation Improves Host Cone Survival in the P23H Rat Model of Retinitis Pigmentosa. . Invest. Ophthalmol. Vis. Sci. 2006;47(13):4803.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : We previously demonstrated that transplantation of rods could limit loss of cones which is responsible for main visual handicap in the retinal degeneration mouse model (rd1). Our purpose is to extend this experimental paradigm to another model of retinal degeneration, the P23H transgenic rat.

Methods: : Heterozygote P23H (line 1) rats (78 and 92 days old) were transplanted subretinally in one eye with retinal sheets (2mm x 4mm) obtained from postnatal 8 day Sprague Drawley rats. After various survival times (240–330 days), transplanted retinas were analysed by immunocytochemistry and histology. We quantified the numbers of retinal cones, using a stereological approach to obtain unbiased samples after immunolabeling with peanut agglutinin (PNA) lectin to label cones and anti–opsin Rho–4D2 antibody to label rods.

Results: : In histological sections and on flat mounted retinas demonstrated the persistence of rods within the grafts in the host retinas. In the grafted retinas, cone numbers were higher 2%–85% than that in mocked treated controls. The rescue of cones was an average of 28% for 24 rats examined. Variance analysis and T test showed statistically significance. Meanwhile, the results showed progressive decrease in cone numbers, both in the controls and the grafted retinas over time.

Conclusions: : The present study suggests that the transplantation of retinal sheets, as photoreceptor sheets, can delay the death of cones in a dominant model of rod–cone degeneration. This observation extends the domain of application of transplantation for the treatment in the human Retinitis Pigmentosa.

Keywords: transplantation • photoreceptors • cell survival 
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