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J. Seo, K.H. Park, D. Kim, Y.J. Kim; The Differences of Histopathology and Expression of Matrix Metalloproteinases in Tenon’s Tissue of Primary Open–Angle Glaucoma and Primary Angle–Closure Glaucoma . Invest. Ophthalmol. Vis. Sci. 2006;47(13):5050.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the histopathologic differences and expressions of matrix metalloproteinases(MMPs) of Tenon’s tissue in primary open–angle glaucoma(POAG) and primary angle–closure glaucoma(PACG).
The POAG or PACG patients, who had received trabeculectomy without history of systemic disease and ocular surgery and retinal vascular disease and uveitis, were prospectively enrolled and their medical records were reviewed for the number of topical eye drops and the period of using topical eye drops. For the control group, Tenon’s tissues were obtained during retinal detachment surgery in the patients who were not glaucoma, without history of ocular surgery. In glaucoma patients, Tenon’s tissue were obtained during the procedure of trabeculectomy and stained with H&E and Masson’s trichrome. For the evaluation of expressions of MMP–1, MMP–2, and MMP–9, immunohistochemistry was done. Total 3 POAG, 3 PACG, and 4 control eyes were evaluated.
The duration of topical anti–glaucoma medication was not significantly different between POAG and PACG group(23.3±22.4 vs 35.3±25.0 months, p=0.513). The mean number of anti–glaucoma medication was not different between them(3.0±1.0 vs 2.7±1.5, p=0.822). Significant increase of subepithelial collagen density and fibroblast density in Tenon’s tissue of both POAG and PACG patients. The fibrosis looked more increased in POAG than in PACG patients under H&E and Masson’s trichrome staining. Compared to the control, MMP–9(p=0.034) was decreased in POAG while MMP–1 (p=0.025) and MMP–9 decreased in PACG(P=0.034). (Mann–Whitney U tests). The levels of MMP–2 in POAG and PACG were not significantly different from those of the control eyes(p=0.102). The levels of MMP–1and MMP–2 in PACG patients were lower than that of POAG patients but not statistically significant (p=0.110).
In POAG patients, the Tenon’s tissue showed increased fibrosis and higher density of collagen than that of PACG patients. The levels of MMP–1 and MMP–2 in PACG patients were lower than in POAG patients. There may be some histopathologic and molecular differences in the Tenon’s tissue between POAG and PACG, which may be confirmed by larger numbers of cases.
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