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B.A. Aird, T.R. McNamara, J.A. Gow, C.K. Song, T.W. Joe, G.A. Baklayan; The Compatibility of Vitrase® Combined With Avastin® . Invest. Ophthalmol. Vis. Sci. 2006;47(13):5260.
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To determine the compatibility of Vitrase® (hyaluronidase for injection) lyophilized, ovine (6200 USP units/vial) and Avastin® (bevacizumab), when combined.
Vitrase (6200 USP units/vial) was reconstituted with 0.54 mL of sterile, unpreserved normal saline (0.9% sodium chloride for injection) and combined with Avastin to obtain concentrations of approximately 34 USP units per 50 µL Avastin, 68 USP units per 50 µL Avastin and 136 USP units per 50 µL Avastin. Sample preparations were visually inspected for precipitation over a period of 4 days. Hyaluronidase activity in the presence and absence of Avastin was determined using a validated turbidometric method. The ability of Avastin to bind vascular endothelial growth factor (VEGF) in the presence and absence of Vitrase was determined using the VEGF ELISA kit (Assay Designs Inc.), supplied with VEGF standard and plates pre–coated with the immobilized human anti–VEGF antibody.
All sample preparations (34 USP units of Vitrase per 50 µL of Avastin, 68 USP units of Vitrase per 50 µL of Avastin and 136 USP units of Vitrase per 50 µL of Avastin) remained free of turbidity over the course of 4 days. Vitrase activity remained unchanged and the ability of Avastin to bind VEGF also remained unchanged for all three sample concentrations of Vitrase.
Various concentrations of Vitrase were found to be compatible when combined with Avastin. There were no visible signs of precipitation over a period of 4 days. Hyaluronidase activity and the ability of Avastin to bind VEGF remained unchanged for all three sample concentrations of Vitrase. In the in vitro setting, the combination of Vitrase and Avastin was found to retain full biological activity and stability for both constituents.
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