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H. Imai, N. Katai, N. Senda, T. Yanagidaira, T. Murata; ßB2–Crystallin Expressed in Retinal Ganglion Cells After Ischemic Reperfusion Injury . Invest. Ophthalmol. Vis. Sci. 2006;47(13):5503.
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© ARVO (1962-2015); The Authors (2016-present)
We previously screened the gene expression profile in retinal ischemia reperfusion injury by using the DNA microarray system and discovered many kinds of ß and γ–crystallin gene were expressed in injured retina. However, the function of ß and γ–Crystallin expressed in extralenticular tissues is unknown. Our purpose is to characterize the ßB2–crystallin induced in retinal ganglion cells after ischemia reperfusion injury.
Transient retinal ischemia for 60 minutes was performed to male Sprague–Dawley rats (200–300g) by increasing the intraocular pressure. Retinas were collected at various time points after reperfusion, and both protein and gene expression levels were measured by using Western blotting and real–time RT–PCR methods, respectively. Localization of ßB2–crystallin proteins in injured retina was determined using the immunohistochemical methods.
On Western blotting, ßB2–crystallin was induced in injured retina from 6 to 24 hours after ischemic insult. The peak time point was at 24 hours after reperfusion. ßB2–crystallin gene expression level was gradually increased after the injury and the peak time point was at 24 hour after the ischemic insult. Immunohistochemical study showed that ßB2–crystallin was expressed mainly in retinal ganglion cells but not in apoptotic cells.
ßB2–crystallin was induced in the retinal ganglion cells after the ischemic insult.
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