Purchase this article with an account.
N. Wang, Q. Lu, L. Wang; Expression of Matrix Metalloproteinases and Inhibitors by Human Choroidal Melanocytes in vitro . Invest. Ophthalmol. Vis. Sci. 2006;47(13):5620.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To investigate the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) by cultured human choroidal melanocytes, so as to implore more physiologic functions of choroidal melanocytes.
Immunocytochemical methods were used to identify human choroidal melanocytes. We detected the quantity of MMPs and TIMPs by Western blot and positioned them by immunofluroescent staining.
In Western blot study using the same amount of total protein, we detected only MMP–2 in normal choroidal melanocytes by every characteristic antibody, while the signals of MMP–1, 3, 9 and TIMP–1, 2 were not found. MMPs were expressed in the cytoplasm of melanocytes by immunofluroescent staining.
Some type of MMPs could be expressed by cultured choroidal melanocytes; while TIMPs could not be expressed.
This PDF is available to Subscribers Only