May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Morphometric Analysis of the Retinal Astroglia in an Ocular Hypertension Model
Author Affiliations & Notes
  • J.–M. Ramirez
    Inst Invest Oftalmol Ramon Castroviejo, Universidad Complutense Madrid, Madrid, Spain
  • J.J. Salazar
    Inst Invest Oftalmol Ramon Castroviejo, Universidad Complutense Madrid, Madrid, Spain
  • A.I. Ramirez
    Inst Invest Oftalmol Ramon Castroviejo, Universidad Complutense Madrid, Madrid, Spain
  • B. Rojas
    Inst Invest Oftalmol Ramon Castroviejo, Universidad Complutense Madrid, Madrid, Spain
  • R. De Hoz
    Inst Invest Oftalmol Ramon Castroviejo, Universidad Complutense Madrid, Madrid, Spain
  • M. Villegas–Perez
    Facultad de Medicina, Laboratorio de Oftalmología, Universidad de Murcia, Murcia, Spain
  • S. Mayor–Torroglosa
    Facultad de Medicina, Laboratorio de Oftalmología, Universidad de Murcia, Murcia, Spain
  • M. Salinas
    Facultad de Medicina, Laboratorio de Oftalmología, Universidad de Murcia, Murcia, Spain
  • M. Vidal–Sanz
    Facultad de Medicina, Laboratorio de Oftalmología, Universidad de Murcia, Murcia, Spain
  • A. Triviño
    Inst Invest Oftalmol Ramon Castroviejo, Universidad Complutense Madrid, Madrid, Spain
  • Footnotes
    Commercial Relationships  J. Ramirez, None; J.J. Salazar, None; A.I. Ramirez, None; B. Rojas, None; R. De Hoz, None; M. Villegas–Perez, None; S. Mayor–Torroglosa, None; M. Salinas, None; M. Vidal–Sanz, None; A. Triviño, None.
  • Footnotes
    Support  ISCIII–FISC03/13. PR48/01–9903
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 1318. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      J.–M. Ramirez, J.J. Salazar, A.I. Ramirez, B. Rojas, R. De Hoz, M. Villegas–Perez, S. Mayor–Torroglosa, M. Salinas, M. Vidal–Sanz, A. Triviño; Morphometric Analysis of the Retinal Astroglia in an Ocular Hypertension Model . Invest. Ophthalmol. Vis. Sci. 2005;46(13):1318.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: To study the effects of chronic ocular hypertension on astrocytes in adult rat retinas. Methods: The intraocular pressure of the left eye in adult Sprague–Dawley rats was chronically elevated by laser photocoagulation (wavelength 532nm, power 500mW, duration 0.25 seconds, spot diameter 100 mm) of the limbus and most of the episcleral veins. Intraocular pressure in both eyes was measured with a Tono–Pen prior to and 1 and 2 weeks after lasering. Retinal whole–mounts were processed by immunohistochemistry (anti–GFAP). The retinal area occupied by astrocytes was determined and analyzed statistically. Results: In the control eyes the star–shaped astrocytes were distributed throughout the retina, forming a network in the nerve fiber layer. The number of astrocytes in the glaucomatous eyes decreased overall. These cells decreased towards the retinal periphery and the decrease was statistically significant. Also, the astrocyes associated with the blood vessels showed higher immunoreactivity that in the controls. Additionally, the Müller cells in these retinas exhibited intense GFAP immunoreactivity. Conclusions: Chronic elevation of the intraocular pressure resulted in retinal ischemia 3 weeks later, which induced hyperreactivity of the retinal macroglia and posterior subsequent loss of astrocytes. These alterations of the glial cells could have induced axonal degeneration.

Keywords: intraocular pressure • retinal glia • ischemia 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×