May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Protection of UV Irradiation–Induced Cornea Epithelial Cell Apoptosis by Caffeine Through Suppression of JNK Activation
Author Affiliations & Notes
  • L. Wang
    Div of Molecular Medicine, Harbor–UCLA Research & Educ Inst, Torrance, CA
  • L. Lu
    Div of Molecular Medicine, Harbor–UCLA Research & Educ Inst, Torrance, CA
  • Footnotes
    Commercial Relationships  L. Wang, None; L. Lu, None.
  • Footnotes
    Support  EY12953 and EY15282
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 2124. doi:
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      L. Wang, L. Lu; Protection of UV Irradiation–Induced Cornea Epithelial Cell Apoptosis by Caffeine Through Suppression of JNK Activation . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2124.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: The purpose of the study is to define the role of molecular interaction between caffeine and UV–induced JNK cascades in promoting corneal epithelial cell survival from UV irradiation. Methods: rabbit and human cornea epithelial cell were cultured in DMEM/F12 medium containing 10% FBS and 5 µg/ml insulin at 370C, 5% CO2. DNA fragmentation and A/O nuclear staining were performed to detect cell death. Western blot, immunoprecipitation and kinase assays were employed to measure UV–induced MAP kinase activity. Results: 1) UV irradiation induced corneal epithelial cell death was prevented by caffeine in a dose–dependent manner. 2) JNK activity activated by UV irradiation was suppressed by caffeine in the similar dose range. 3) The inhibitory effect of caffeine on JNK activity was not affected by alterations of either cAMP levels or intracellular Ca++ concentrations. 4) Inhibition of PI3K pathway using wortmannin had no effect on caffeine mediated protection. 5) Theophylline, an adenosine receptor antagonist, mimics the role of caffeine in UV–induced JNK activation. 6) Neither caffeine nor theophylline affects EGF– and sorbital–induced ERK and p38 activation, respectively. Conclusions: Caffeine is the world’s most popular consumption because of present in coffee, tea, chocolate, soft drinks, and pain and appetite suppressant. We have found a new role of caffeine that promotes survival of cornea epithelial cells from UV irradiation–caused damage by specific suppression of JNK activation.

Keywords: apoptosis/cell death • signal transduction • pharmacology 
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