May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Role of TGF–ß Signaling in Wound Healing of Corneal Epithelium
Author Affiliations & Notes
  • K. Terai
    Ophthalmology, University of Cincinnati, Cincinnati, OH
  • Y. Hayashi
    Ophthalmology, University of Cincinnati, Cincinnati, OH
  • T.–I. Chikama
    Ophthalmology, University of Cincinnati, Cincinnati, OH
  • N. Terai
    Ophthalmology, University of Cincinnati, Cincinnati, OH
  • W.W. Kao
    Ophthalmology, University of Cincinnati, Cincinnati, OH
  • Footnotes
    Commercial Relationships  K. Terai, None; Y. Hayashi, None; T. Chikama, None; N. Terai, None; W.W. Kao, None.
  • Footnotes
    Support  NIH Grant EY13755, EY14207; RPB; OLERF
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 2144. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      K. Terai, Y. Hayashi, T.–I. Chikama, N. Terai, W.W. Kao; Role of TGF–ß Signaling in Wound Healing of Corneal Epithelium . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2144.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: To examine the roles of TGF–ß signaling pathways in regulating cell migration and proliferation of the healing of corneal epithelium debridement. Methods: TGF–ß type II receptor (TbR2) floxed mice were bred with Krt12–Cre mice to generate bitransgenic mice in which the TGF–ß receptor 2 gene was disrupted selectively in the corneal epithelial cells. Corneal epithelial debridement (2 mm diameter) was created in 2–month–old bitransgenic Krt12Cre/CreTbR2f/f mice and their littermates as controls Krt12Cre/CreTbR2f/+ and Krt12Cre/CreTbR2+/+. The healing of corneal epithelium defect was assessed by fluorescein staining at different intervals of debridement. The experimental mice were administrated by i.p. injection of BrdU two hours prior to sacrifice to determine cell proliferation. Immunohistochemistry was performed to elucidate potential signal transduction molecules involved. Results: The corneal epithelium of Krt12Cre/CreTbR2f/f mice lost TbR2 as judged by western blot analysis with anti–TbR2 antibodies and exhibited delayed healing of debridemnt, in comparison to control littermates that were heterozygous floxed and wild typeTbR2. The naïve uninjured corneal epithelium of Krt12Cre/CreTbR2f/f mice exhibited higher cell proliferative activities than controls. Corneal epithelium debridement caused cessation of epithelial cell proliferation in all three groups of experimental mice in 6–12 h. Immunohistochemistry using anti–phospho–p38 revealed a significant decrease of phosphorylation of p38 in 6–12 h of debridement. Conclusions: Our results indicate that the TGF–ß signals play a critical role in the corneal epithelial wound healing, especially in the migration of corneal epithelial cells.

Keywords: wound healing • cornea: epithelium • transgenics/knock-outs 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×