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Y. Hayashi, C.–Y. Liu, D.Y. Weng, W.W. Kao; Role of cJun in Eyelid Morphogenesis during Mouse Embryonic Development . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2177.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: We previously reported that the reduction of cJun activation in periocular mesenchymal cells resulting from interrupted TGF–α gradient by excess biglycan perturbed eyelid morphogenesis of Kera–Bgn transgenic mice. In present study, we further examined the role of cJun in eyelid morphogenesis. Methods: The Cre–loxP system was adapted for eyelid–specific gene ablation. Keratocan promoter (Kera5) containing 3.2 kb 5’ flanking sequence, exon 1 and intron 1 of Kera gene was used to prepare transgenic mouse lines expressing Cre. KC9, one of 22 Kera5–Cre founder mouse lines, were crossed with ROSA26R (LacZ reporter mice) and cJun–floxed mice (cJunf/f). The distribution of cells derived from periocular mesenchyme was mapped by determining ß–galactosidase activities in eyelid with whole mount X–gal staining. The effect of cJun on eyelid morphogenesis was examined by histology. Results: ß–Galactosidase activity was detected in most, if not all eyelid stromal cells, suggesting that they are derived from periocular mesenchyme. The enzyme activity was also detected in tarsal muscle, but absent in orbicularis oculi. KC9 mouse line was mated with cJun– floxed mice to prepare KC9/cJunf/f bitransgenic mice. Histology examination revealed that the KC9/cJunf/f mice had thinner eyelid with fewer stromal cells than that of KC9/cJunf/+ (heterozygous cJun– floxed alleles) and wild type mice. However, the KC9/cJunf/f did not show premature eye open phenotype.Conclusions:cJun signaling has important role for normal eyelid morphogenesis. The ablation of cJun in periocular mesenchymal cells may alter cell migration, proliferation and apoptosis required for normal eyelid morphogenesis during embryonic development.
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