May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Promotion by Latanoprost of Collagen Gel Contraction Mediated by Human Corneal Fibroblasts
Author Affiliations & Notes
  • R. Yanai
    Biomolec Recognition & Ophthal, Yamaguchi Univ School of Med, Ube City, Japan
  • Y. Liu
    Biomolec Recognition & Ophthal, Yamaguchi Univ School of Med, Ube City, Japan
  • Y. Lu
    Biomolec Recognition & Ophthal, Yamaguchi Univ School of Med, Ube City, Japan
  • S. Hirano
    Biomolec Recognition & Ophthal, Yamaguchi Univ School of Med, Ube City, Japan
  • T. Sagara
    Biomolec Recognition & Ophthal, Yamaguchi Univ School of Med, Ube City, Japan
  • T.–I. Chikama
    Biomolec Recognition & Ophthal, Yamaguchi Univ School of Med, Ube City, Japan
  • T. Nishida
    Biomolec Recognition & Ophthal, Yamaguchi Univ School of Med, Ube City, Japan
  • Footnotes
    Commercial Relationships  R. Yanai, None; Y. Liu, None; Y. Lu, None; S. Hirano, None; T. Sagara, None; T. Chikama, None; T. Nishida, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 2186. doi:
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      R. Yanai, Y. Liu, Y. Lu, S. Hirano, T. Sagara, T.–I. Chikama, T. Nishida; Promotion by Latanoprost of Collagen Gel Contraction Mediated by Human Corneal Fibroblasts . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2186.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Measurement of intraocular pressure depends on the shape and thickness of the cornea. The possible effects of the antiglaucoma drugs latanoprost, timolol maleate, and pilocarpine on the contraction of corneal tissue were examined with the use of three–dimensional cultures of human corneal fibroblasts. The effects of these drugs on collagen degradation as well as their potential cytotoxicity were also examined with this system. Methods:Human corneal fibroblasts (CFs) were cultured in three–dimensional gels of type I collagen and in the presence of medium containing various concentrations (0.01 to 1000 µM) of latanoprost, timolol maleate, or pilocarpine. Collagen gel contraction was evaluated by daily measurement of the gel diameter for 3 or 4 days. The extent of collagen degradation was determined by measurement of the amount of hydroxyproline generated by acid–heat hydrolysis of the culture supernatants. The release of lactate dehydrogenase from the cells was determined as an index of drug cytotoxicity. Results:Latanoprost promoted CF–mediated collagen gel contraction in a dose– and time–dependent manner, whereas timolol maleate and pilocarpine did not exhibit such an effect. Latanoprost and timolol maleate, but not pilocarpine, showed a cytotoxic effect at the highest concentration (1000 µM) tested. Neither latanoprost, timolol maleate, nor pilocarpine affected collagen degradation by human CFs at the concentrations examined. Conclusions: Among the antiglaucoma drugs investigated, only latanoprost promoted CF–mediated collagen gel contraction. Latanoprost may thus affect corneal shape through this effect on CFs.

Keywords: cornea: stroma and keratocytes • cornea: basic science • wound healing 
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