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E. Medof, E. Cocuzzi, T. Sakuta, M. Jacobs, D. Bardenstein, J.H. Lass, F. Lin; Essential Role of the Decay Accelerating Factor in Protecting the Eye During Bacterial Keratitis . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2629.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:Corneal and conjunctival epithelia express among the body’s highest levels of decay accelerating factor (DAF), an intrinsic surface regulator that shields self cells from autologous complement mediated injury, but its physiological relevance on the ocular surface is unstudied. As one way to address this, we compared the severity of Pseudomonas keratitis in Daf1–/– mice (devoid of ocular Daf protein) to that in their Daf1+/+ littermates. Methods: Eyes were inoculated with Pseudomonas aeruginosa PAK (which causes chronic keratitis), Paer–1 [which causes contact lens–induced red eye (CLARE)], or with random clinical isolates from patients with varying disease severity, and after 24–72 hr, eyes were enucleated and examined for corneal polymorphonuclear cell (PMN) infiltration, anterior chamber pathology, and complement deposition. Because DAF is present on PMN as well as the ocular surface, parallel studies were done with Daf1–/– and Daf1+/+ controls transplanted with marrow from Daf1+/+ animals. Results: In all cases, Daf1–/– mice exhibited dramatically increased corneal PMN infiltration, suffered markedly more severe corneal damage, and showed greater C3b deposition than Daf1+/+ mice. Daf1–/– mice and their Daf1+/+ littermates transplanted with Daf1+/+ marrow (to exclude effects of Daf deficiency on PMN) showed parallel differences to those in untransplanted Daf1–/– and Daf1+/+ mice. Conclusions: Corneal/conjunctival DAF is essential to circumvent ocular surface injury during bacterial infections. By extrapolation, DAF’s activity is important to protect eyes in other conditions involving complement activation.
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