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J.–S. Gwon, H.–J. Kim, K.–R. Choi, S.–J. Oh, M.–H. Chun; Abstract Title: Retinal Müller Cell Survival in Early Stages of Retina Ischemia–Reperfusion Injury in Rats via the Effect of STAT 3 Pathways . Invest. Ophthalmol. Vis. Sci. 2005;46(13):2973.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To investigate the response of Müller cells in the early stages of ischemia– reperfusion injury. Methods: The intraocular pressure (IOP) was raised to 90–120 mmHg for 60 min, and the animals were sacrificed after 1 d, 3 d, 1 wk, 2 wk, and 4 wk of reperfusion. Immunocytochemistry using antisera against STAT 3, p–STAT 3 and ciliary neurotrophic factor (CNTF) or glutamine synthase (GS), a specific marker for Müller cell, were applied, and apoptotic cell death was determined by a modified TUNEL technique. Western blotting using anti–STAT 3 and p–STAT 3 antisera were applied, and double labeling using antisera against STAT 3, p–STAT 3 and GS or CNTF were performed. Results: STAT 3 and p–STAT 3 immunoreactivity was localized to most of all Müller cells. In addition, double labeling using antisera against STAT 3, p–STAT 3 and GS or CNTF demonstrated that these two antigens were expressed in same Müller cells. STAT 3 and p–STAT 3 labeled Müller cells did not show positive staining by TUNEL method. Conclusions: : Our findings demonstrate that most of Müller cells survive by STAT 3 pathway and suggest that STAT 3 produced and released by Müller cell may play an important role in the pathogenesis of ischemic injury in the rat retina.
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