May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Evaluation of Basics Fibroblast Growth Factor (bFGF) Level in Pig Eyes That Underwent Surgical Adypocytes Implant Under the Scleral Plane
Author Affiliations & Notes
  • S.Z. Scalinci
    Pathophysiological Optics, University Bologna, Bologna, Italy
  • L. Scorolli
    Pathophysiological Optics, University Bologna, Bologna, Italy
  • A. Meduri
    Department of Ophthalmology, University of Messina, Messina, Italy
  • M. Morara
    Pathophysiological Optics, University Bologna, Bologna, Italy
  • S. Degli Esposti
    Pathophysiological Optics, University Bologna, Bologna, Italy
  • R.A. Meduri
    Pathophysiological Optics, University Bologna, Bologna, Italy
  • Footnotes
    Commercial Relationships  S.Z. Scalinci, None; L. Scorolli, None; A. Meduri, None; M. Morara, None; S. Degli Esposti, None; R.A. Meduri, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 3118. doi:
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      S.Z. Scalinci, L. Scorolli, A. Meduri, M. Morara, S. Degli Esposti, R.A. Meduri; Evaluation of Basics Fibroblast Growth Factor (bFGF) Level in Pig Eyes That Underwent Surgical Adypocytes Implant Under the Scleral Plane . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3118.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To evaluate the level of bFGF release in pig eyes after surgical adipocytes implant under the scleral plane compared to bFGF level in normal eyes. Methods: 18 pigs, mean age 0.5±0.02 years old. 6 pigs underwent adipocytes implant in both eyes (group A), 6 pigs underwent implant in only one eye, chosen in a randomized way (group B), and 6 pigs did not undergo any surgical operation (group C). After 4 months from the implant 6 pigs (2 from group A, 2 from B and 2 from C) were sacrified with mechanical procedure in order to preserve retinal tissue. The same procedure was performed after 8 months and after 12 months. Expianted eyes were divided into two groups: group 1, that consisted of operated eyes, and group 2, that consisted of non–operated eyes. We performed on all expianted eyes bFGF dosage by means of immunoistochimical technique (immunoassay Kit GFG basic KHG0021, BioSource International Inc., Caramillo, California, USA). The research was conducted in compliance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research. Results: We evaluated levels of endobulbar bFGF in vitreal fluid (50 µl). After 4 months bFGF levels were 7.76±0.64pg/ml in group 1, and 0.82±0.16pg/ml in group 2. After 8 months bFGF levels were 6.39±0.72pg/ml in group 1, and 0.75±0.26pg/ml in group 2. After 12 months bFGF levels were 7.21±0.52pg/ml in group 1, and 0.71±0.22pg/ml in group 2. The difference between bFGF levels in the two group proved to be statistically significant in all of the three evaluations (p < 0.05, Wilcoxon Statistical Analysis). This can demonstrate good tropism and good vascularization of peduncle of orbital flat implanted. Conclusions: After surgical adipocytes implant, an increase of intraocular bFGF level can be observed.

Keywords: growth factors/growth factor receptors • retina • immunohistochemistry 
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