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Y.–F. Shih, I.–J. Wang, Y.–C. Hou, L. Lin; The Association of Single Nucleotide Polymorphism at the Decorin, Lumican and DSPG3 Genes With Susceptibility to High Myopia . Invest. Ophthalmol. Vis. Sci. 2005;46(13):3209.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To evaluate the relationship between two single nucleotide polymorphisms (SNPs) of decorin, lumican, and DSPG3 genes and high myopia. Methods: We collected 89 adult patients with high myopia (<–10.0 D) and 111 controls with –1.5 to 0.5 D to study the association of the decorin, lumican and DSPG3 genes with high myopia. All of these subjects were free of ocular diseases except myopia and other systemic genetic diseases. Genotyping was performed by direct sequencing after PCR amplification of chromosomal DNA. Allele frequencies were tested for Hardy–Weinberg equilibrium (HWE). The Χ2 or Fishder test was conducted to investigate the genotypic and allelic distribution between high myopia and control groups. Results: The genotyping successful rate was 98%. The genotype distribution was not deviated from HWE. For decorin gene (rs2070985), there were 66 CC, 20 CG and 3 GG in case group; 82 CC, 22 CG and 4 GG in control group. For lumican gene (rs3741835), there were 49AA, 34 AG and 6 GG in case group; 48 AA, 50 CG and 13 GG in control group. For DSPG3 gene (rs1920751), there were 5 AA, 24 AG and 60 GG in case group; 7 TT, 35 TG and 70 GG in control group. There was significant difference for lumican gene between two subgroups with a p value of 0.031. However, there was no significant difference for decorin and DSPG genes. Conclusions: Our results reveal that the extracellular matrix glycosaminoglycan, especial lumican, plays an important role in the development of high myopia.
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