May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
LASEK Induces Less Corneal Wound Healing and Better Transmittance Than PRK in the Iber Braun Hens Corneal Model
Author Affiliations & Notes
  • J.M. Merayo–Lloves
    Ophthalmology IOBA,
    Universidad de Valladolid, Valladolid, Spain
  • H.A. Martinez–Osorio
    Ophthalmology IOBA,
    Universidad de Valladolid, Valladolid, Spain
  • T. Blanco–Mezquita
    Ophthalmology IOBA,
    Universidad de Valladolid, Valladolid, Spain
  • C. Martinez–Garcia
    Cell Biology,
    Universidad de Valladolid, Valladolid, Spain
  • S. Mar–Sardaña
    Physics Applied,
    Universidad de Valladolid, Valladolid, Spain
  • R.M. Torres
    Ophthalmology IOBA,
    Universidad de Valladolid, Valladolid, Spain
  • Footnotes
    Commercial Relationships  J.M. Merayo–Lloves, None; H.A. Martinez–Osorio, None; T. Blanco–Mezquita, None; C. Martinez–Garcia, None; S. Mar–Sardaña, None; R.M. Torres, None.
  • Footnotes
    Support  H. Martinez–Osorio, Carolina Foundation, Ministry of foreign affairs, Spain
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 4360. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      J.M. Merayo–Lloves, H.A. Martinez–Osorio, T. Blanco–Mezquita, C. Martinez–Garcia, S. Mar–Sardaña, R.M. Torres; LASEK Induces Less Corneal Wound Healing and Better Transmittance Than PRK in the Iber Braun Hens Corneal Model . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4360.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: To compare corneal wound healing and transmittance after laser subepithelial keratomileusis (LASEK) and photorefractive keratectomy (PRK) in the Iber Braun hens corneal model. Methods: LASEK surgery (–6 D, optical zone 6.0 mm, 67 µm) was performed on Iber Braun hens (2,5 kg) corneal model. Iber Braun hens corneal epithelium was exposed to 20% ethanol in BBS for 40 seconds using as the reservoir a 7.5 mm trephine. The epithelium was detached by the technique "in the bag" and then treated with an excimer laser (Summit apex plus). The epithelial flap was repositioned. Morphologic changes were evaluated by optic (H–E) and electronic microscopy examination. Proliferation and apoptosis were studied at 24 and 72 hours. The transmittance and scattering measurements were done with a scatter meter constructed in the Optic’s Department (University of Valladolid) at 15 and 30 days. Results: LASEK corneas showed TUNEL positive cells in the basal layer of the epithelial flap. In the stroma, there are less keratocyte apoptosis in LASEK than PRK corneas at 24 hours, and both of them decreased at 72 hours but remained higher after PRK than LASEK. Keratocyte proliferation rate at 72 hours were higher in the two superior thirds of the corneas in the PRK than the LASEK model. LASEK corneas had less scattering and better transmittance than PRK corneas at 15 and 30 days. Conclusions: LASEK induces less stromal scarring compared to PRK in the Iber Braun hens corneal model. LASEK corneas have better transmittance than PRK corneas. Iber Braun hens offer a very good model of LASEK corneal refractive surgery.

Keywords: cornea: basic science • wound healing • refractive surgery 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×