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D.B. Denham, J.–M. Parel, P. Lamar, A.C. Acosta, A. Abri, W. Lee, S. Dubovy, F. Manns, A. Ho, R. Augusteyn; BPEI–MIYAKE–APPLE METRIC SYSTEM FOR ANALYSIS OF CRYSTALLINE, PSEUDOPHAKIC AND REFILLED LENSES AND LENS EPITHELIUM PROLIFERATION . Invest. Ophthalmol. Vis. Sci. 2004;45(13):314.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:To design a system to measure and compare the physical dimensions of intact explanted crystalline lenses and pseudophakic lenses and quantify lens epithelium proliferation. Methods: The system consists of a stand with a fiber optic illuminator located in its base and a 5cm diameter discoid platen located 60mm from the fiber plane, a 5 to 30 X motorized zoom operation microscope equipped with a built–in 0o coaxial illuminator, a 30o slit–illuminator and a 3MP digital camera connected to a PC with image processing software. The platen is equipped with a ring–locking mechanism. Fresh human, monkey and rabbit globes were used. After removal of conjunctival, fatty and muscle stump tissues, a rigid ring custom machined to fit the globe scleral surface at 2mm from the limbus is bonded using cyanoacrylate, placed on the platform and locked. The posterior pole is sectioned, a manual vitrectomy performed, and the ring is inverted 180o, and the cornea is removed 0.5mm below the limbus. The bonded ring prevents collapse of the eye section which remains undisturbed during manipulation allowing the tissue section to be flipped and examined on both sides. After digital photography of the iris anterior and posterior surfaces, the iris is removed to give a full view of the anterior capsule surface and anterior zonular apparatus. Image formation analysis and light transmission are performed and the ring–tissue assembly fixed as a unit for LM, TEM or SEM. Results: The system was validated in 5 eyes. It allowed precise metric measurement of crystalline lenses, capsulorhexis shape and surface, IOL centration, measurement of lens diameter, thickness and IOL position with respect to the iris plane, surface and thickness measurement of ACO and PCO and measurement of the lens light transmission and image formation. Conclusions:The BPEI–Miyake–Apple metric lens morphology system is useful for the analysis of crystalline, pseudophakic and Phaco–Ersatz lenses. CR: NONE. Support: NIH grant EY14225; Florida Lions Eye Bank; Vision CRC, Sydney Australia; Fight for Sight; Research to Prevent Blindness, NY, NY; Henri and Flore Lesieur Foundation, Palm Beach, FL.
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