May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
A Comparative Evaluation of Laser–Induced Choroidal Neovascularization in the Cynomolgus Monkey
Author Affiliations & Notes
  • M.C. Wills
    Discovery and Development Services, Sierra Division, Charles River Laboratories, Sparks, NV
  • Footnotes
    Commercial Relationships  M.C. Wills, Charles River Laboratories E.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 466. doi:
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      M.C. Wills; A Comparative Evaluation of Laser–Induced Choroidal Neovascularization in the Cynomolgus Monkey . Invest. Ophthalmol. Vis. Sci. 2004;45(13):466.

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Abstract

Abstract: : Introduction:The primate choroidal neovascularization (CNV) model has been used for several studies in this laboratory. Fluorescein angiography data from these studies was evaluated. The purpose of this study was to evaluate the relationship of animal characteristics (age, sex, study history, origin and weight) and location of laser lesion to development of CNV. Methods:Forty–six cynomolgus monkeys were evaluated. Laser lesions (9–spot pattern) were induced in the perimacular region with a green laser (532 nm, spot size 75–100 µm, power 450–900 mW, exposure time 0.1–0.2 s). A vaporization bubble indicated disruption of Bruch’s membrane. Lesions that developed a clear luminescent circle during fluorescein angiography were counted as CNV. Animals were divided into prepubertal (2–5 years) and adult (over 5). The prepubertal group was subdivided into younger (2–3.5 years) and older (3.6–5 years). Animals were divided into groups of 2–3.5 kg and 3.6–5.2 kg. CNV incidence was compared for males versus females, naive versus non–naive and Chinese versus Indonesian. Results:Twelve animals had been treated with a test article and did not develop CNV in either eye and were excluded from evaluation. Twenty–five animals aged 2–5 years developed CNV. All 10 animals aged 2–3.5 developed CNV, while 15/17 animals aged 3.6–5 years developed CNV. Six animals over the age of 5 developed CNV. Eighteen animals that weighed 2–3.5 kg developed CNV. Thirteen animals in the 3.6 to 5.2 weight range developed CNV. Twenty–six males developed CNV, while all five females develop CNV. Eighteen non–naïve animals developed CNV. Thirteen naïve animals developed CNV. Twenty–six Chinese cynomolgus monkeys developed CNV; five Indonesian cynomolgus monkeys developed CNV. Lesions placed adjacent to or directly above the macula and nasal or superio–nasal to the macula developed CNV most often. CNV developed at least once in each of the 9 lesions. Conclusions:There was no evidence of age– or weight–related differences in the propensity to develop CNV. Insufficient numbers of females and Indonesian animals were available to conclusively determine sex– or origin–related differences. Non–naïve animals developed CNV at a similar rate as naïve animals. Laser lesions placed adjacent to or directly above the macula and in lesions placed nasal and superio–nasal to the macula appear to be most likely to develop CNV.

Keywords: choroid: neovascularization • age–related macular degeneration • Bruch's membrane 
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