May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Expression of Fractalkine and CX3CR1 in Expreimental Autoimmune Anterior Uveitis
Author Affiliations & Notes
  • I.–M. Fang
    Ophthalmology, Chung–Hsiao Municipal Hospital,Taipei City, Taipei, Taiwan Republic of China
  • C.–H. Yang
    Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan Republic of China
  • C.–P. Lin
    Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan Republic of China
  • C.–M. Yang
    Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan Republic of China
  • M.–S. Chen
    Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan Republic of China
  • Footnotes
    Commercial Relationships  I. Fang, None; C. Yang, None; C. Lin, None; C. Yang, None; M. Chen, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 549. doi:
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      I.–M. Fang, C.–H. Yang, C.–P. Lin, C.–M. Yang, M.–S. Chen; Expression of Fractalkine and CX3CR1 in Expreimental Autoimmune Anterior Uveitis . Invest. Ophthalmol. Vis. Sci. 2004;45(13):549.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To demonstrate the expression and location of fractalkine and its receptor, CX3CR1, in iris/ciliary body and thus establish their roles in experimental autoimmune anterior uveitis, an animal model of human acute anterior uveitis. Methods: Uveitis was induced with the injection of melanin–associated antigen into the left footpad. At defined time points, fractalkine and cognate receptor CX3CR1 mRNA expression were semiquantified by using a RT–PCR. The concentration of fractalkine in aqueous humor was determined by ELISA. The cellular source of fractalkine was demonstrated by using immunohistochemical stain. Results: Fractalkine mRNA increased expression on day 3 and reach its peak on day 11, preceding the onset of clinical disease. Meanwhile, CX3CR mRNA was upregulated on day 11, concurrent with peak expression of fractalkine. Significantly increased levels of fractalkine could also be measured in the aqueous humor since days 11 and last for the whole clinical disease. Immuhistochemical staining revealed that fractalkine was prominently expressed on endothelial cells of vessel wall in iris/ciliary body. Conclusions: These findings suggested that fractalkine might be functional in leukocyte trafficking to inflamed iris/ciliary body and thus play a pathogenic role in the genesis of experimental autoimmune anterior uveitis.

Keywords: uveitis–clinical/animal model • autoimmune disease • uvea 
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