Purchase this article with an account.
S. Tanimoto, T. Kanamoto, T. Yokoyama, N. Noma, H. Aoyama, H.K. Mishima; Proteomics analysis for the screening of retinal development factors . Invest. Ophthalmol. Vis. Sci. 2004;45(13):673.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: To analyze the mechanism of retinal development. Methods: Two protein lysate samples, that embryonic day 7 and 11, were prepared from chick embryo retina. Total retina lysates were electrophoresed on two–dimensional SDS page gels, and isolated protein spots on gels were detected by silver staining. The change of all spots expressions were differentially displayed, dependent on embryonic day, by the software ( PD–Quest : BioRad). The target spots, protein expressions were dramatically changed, were selected, and the proteins of targets were identified by mass –spectrometry ( Q–STAR : Applied Biosystems ). Moreover, the functional analysis of target proteins was tried out, using three dimensional tissue culture of chick embryo retina. Results: Many target proteins, as suspected retinal differentiation factors, were detected, and we made clustering analysis of target proteins. Conclusions: Proteomics analysis of chick embryo retina is succeeded, and we suggest that the target proteins may act on retinal development.
This PDF is available to Subscribers Only