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A.W. Siu, B.B. Tam, E.Y. Lee, A.F. Lam; Pinoline protects the retina from lipid peroxidation . Invest. Ophthalmol. Vis. Sci. 2004;45(13):734.
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Purpose: Pinoline is a pineal indoleamine naturally found in the retina. This study compared the effects of pinoline and vitamin E on the copper(I)–induced retinal lipid peroxidation (LPO). Methods: Procine retinal homogenates were mixed with 120µM copper(I) solution. The mixtures were co–incubated with various concentrations of pinoline or trolox (water–soluble vitamin E analogue) at 37°C for 60min. The amounts of malondialdehyde (MDA) and protein were then assayed to quantify the LPO. Results: Copper (I) ions significantly increased the MDA concentration in the retinal homogenates (p<0.0007). Both pinoline and trolox significantly suppressed MDA in a dose–dependent manner (p<0.0001) and their effects were significantly different (p=0.004). The concentrations that inhibited 50% of LPO were 0.24 mM and 0.68 mM for pinoline and trolox respectively. Conclusions: Pinoline demonstrated an anti–LPO potency of 2.8 times compared with trolox. The results support an anti–oxidative role of pinoline in the retina.
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