May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Anesthesia Affects Mouse ERG and Blood Glucose
Author Affiliations & Notes
  • E.T. Brown
    Center of Vision Research,
    SUNY Upstate Medical University, Syracuse, NY
  • Y. Umino
    Center of Vision Research,
    SUNY Upstate Medical University, Syracuse, NY
  • E. Solessio
    Center of Vision Research,
    SUNY Upstate Medical University, Syracuse, NY
  • T. Loi
    Center of Vision Research,
    SUNY Upstate Medical University, Syracuse, NY
  • R. Quinn
    Laboratory Animal Resources,
    SUNY Upstate Medical University, Syracuse, NY
  • R. Barlow
    Center of Vision Research,
    SUNY Upstate Medical University, Syracuse, NY
  • Footnotes
    Commercial Relationships  E.T. Brown, None; Y. Umino, None; E. Solessio, None; T. Loi, None; R. Quinn, None; R. Barlow, None.
  • Footnotes
    Support  NEI, NIMH, Research to Prevent Blindness and Lions of Central NY
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 742. doi:
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      E.T. Brown, Y. Umino, E. Solessio, T. Loi, R. Quinn, R. Barlow; Anesthesia Affects Mouse ERG and Blood Glucose . Invest. Ophthalmol. Vis. Sci. 2004;45(13):742.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Investigate the effect of common anesthetics on both the ERG and blood glucose of mice. Methods: Fed and fasted C57/BL6J mice were injected I.P. with one of four anesthetics (see below). Under dark–adapted conditions their pupils were dilated with 1% tropicamide and corneas were moistened with methylcellulose (Moisture Eyes, Bausch Lomb). A unipolar Burian Allen mouse ERG electrode was placed on the cornea with reference in the mouth. ERGs were recorded (100gain, 0.3–1000Hz) in response to 10–ms flashes delivered under computer control (P–Clamp, Axon Instruments) from a cluster of 520nm LEDs placed 1cm from the cornea (Maximum intensity of 230µW /cm2). The animal was maintained near 380C with a heating pad. Using a OneTouch Ultra glucose meter blood glucose (BG) levels were measured from tail blood before anesthesia, 15min and 60min after injection of anesthetic. Results:The effects of four anesthetics and saline control after 60min are:  

Doubling blood glucose (137 to 267mg/dl) causes about a 50% increase in ERG b–wave. Conclusions:Commonly used Ketamine/Xylazine anesthesia maximally increases BG and ERG b–wave. Nembutal has minimal effects. The mechanism of anesthetic action is not known, but the correlation of BG with ERG b–wave has been detected in cats and humans. We suggest that some anesthetics cause an increase in blood glucose that in turn increases retinal sensitivity. CR: None Support: NEI, NIMH, Research to Prevent Blindness and Lions of Central NY.

Keywords: electroretinography: non–clinical • metabolism • retina 
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