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J.T. Blanco–Mezquita, J.M. Merayo–LLoves, M.C. Martínez–García, R.M. Torres, M. González–Parra, S. Bonini, A. Lambiase; TOPICAL ADMINISTRATION OF NERVE GROWTH FACTOR MODULATE CORNEAL WOUND HEALING AFTER REFRACTIVE SURGERY IN AN ANIMAL MODEL. . Invest. Ophthalmol. Vis. Sci. 2004;45(13):1419.
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Purpose: Nerve growth factor (NGF) is produced, stored and released by the epithelium cells, keratocytes and endothelium cells in normal and injury cornea. Recent clinical report demonstrates that topical nerve growth factor treatment in patient with neurotrophic ulcers improve epithelial and stromal wound healing. The purpose of this research is to evaluate in an animal model of photorefractive keratecmony with excimer laser (PRK) the effectiveness of topical corneal application of nerve growth factor (NGF). Methods: Iber Brown hens underwent PRK. Animal were divided into different groups treated with group A topical administration of 0,2% NGF, group B balanced salt solution (BSS) and group C received no treatment. Eyes were exenterated at sequential time points. Sections were stained with H–E and examined under light microscopy. Proliferation was evaluated by immunohistochemistry with Anti–BrdU and TUNEL techniques were used for apoptosis detection. Results: NGF treated corneas (group A) restore epithelium in the first hours and faster than the other groups (B and C). More proliferation was detected in the epithelium and stroma of NGF treated corneas compared with BBS and negative control. Regarding apoptosis NGF treated corneas show less apoptosis than non–treated corneas (Group B and C). Conclusions: NGF modulates corneal wound healing after PRK, inducing a fast recovery of corneal epithelium integrity, increasing the proliferation markers and decreasing keratocytes apoptosis. This model could be used also to study corneal transparency, scattering and aberrations and to develop new therapeutic strategies for complications after corneal refractive surgery.
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