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L.P. Schmidt, R.W. Beuerman; Comparison of Gatifloxacin and Moxifloxacin in Healing of a Linear Incision in the Rabbit Cornea . Invest. Ophthalmol. Vis. Sci. 2004;45(13):1427.
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Purpose: To evaluate the effects of the 2 commercially available ophthalmic solutions of gatifloxacin and moxifloxacin on corneal wound integrity in a rabbit model of corneal surgical wounds Methods: A 6 mm linear incision was made in the eyes of 12 NZW rabbits, and the wounds were closed with two 10–0 nylon sutures. Eyes were then treated with either gatifloxacin ophthalmic solution 0.3% (Zymar, Allergan, Inc., Irvine, CA; n = 6) moxifloxacin ophthalmic solution 0.3% (Vigamox, Alcon, Inc., Fort Worth, TX; n = 6) administered as recommended in the respective package inserts. Contra–lateral eyes served as controls, which were dosed with BSS at a dosing frequency that was the same as that used for each drug. White–light confocal microscopy observations were carried out 1, 4 and 7 days after wounding. Rabbits were euthanized 7 days after wounding and all the corneas were collected and prepared for light and transmission electron microscopy (TEM). The structural analysis was carried out in a blinded fashion. Results:Confocal microscopy of penetrating linear wounds in rabbit corneas suggested that moxifloxacin treatment resulted in less stromal healing at 7 days. Histology verified this observation. The epithelial plug was expressed more quickly with stromal closure after gatifloxacin treatment and renewal of mature epithelial organization. Both antibiotics slowed the process of stromal closure when compared to control treated wounds. Sections from control corneas revealed essentially normal structure along the basal lamina. TEM showed that moxifloxacin treated corneas had protein precipitation just below the basal lamina, which appeared to be thin with anchoring filaments nearly absent. Protein precipitation below the basal lamina was seen consistently outside the wound within 2–3 mm of the incision for moxifloxacin while the gatifloxacin treated wounds appeared similar to the contra–lateral controls. Gatifloxacin resulted in significantly less keratocyte response and stromal disorganization. Conclusions: Gatifloxacin caused significantly less disruption in corneal wound integrity than did moxifloxacin as determined by light microscopy, histology, and TEM.
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