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K. Renganathan, R. Collier, X. Gu, R.G. Salomon, M. Kapin, J.G. Hollyfield, J.W. Crabb; Similarities in Oxidative Damage from AMD and Retinal Light Damage . Invest. Ophthalmol. Vis. Sci. 2004;45(13):1795.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To screen for similarities in oxidative stress mechanisms caused by retinal light damage and age–related macular degeneration (AMD) using rats and in vivo blue light exposure. Methods: Albino rats were dark adapted for 24 hrs. Control rats (N=10) remained in the dark while experimental rats (N=10) were exposed to blue light (220 fc) for 6 hours. Rats were sacrificed immediately following light treatment. Retinas were isolated, immediately washed with antioxidants and frozen at –80ºC until analysis. Lipids were extracted with chloroform/methanol and proteins were solubilized with SDS. Western analysis was used to screen for oxidative protein modifications. Results: Anti–carboxyethylpyrrole and anti–nitrotyrosine immunreactivities were significantly greater after 6h light exposure compared with control animals maintained in the dark. Carboxyethylpyrrole (CEP) adducts are derived from oxidation of docosahexaenoyl lipids (DHA–PC) and nitrotyrosine is generated from reactive nitrogen oxide species. Conclusions: Current results are consistent with our observations that green light stimulates in vivo protein nitration (2002 Mol. & Cell. Proteomics 1, 293) and CEP modifications in rat retina (2003 ARVO abstract 5129). Notably, AMD drusen/Bruch's membrane and light damaged rat retina both contain increased amounts of CEP adducts (2002 Proc Natl Acad Sci USA 99, 14682) and crystallin (2003 Exp Eye Res 76, 131). Rodent light damage models may be useful for validating therapies for AMD. CR: Y. Supported in part by NIH grants EY06603, EY14239, EY14240, GM21249, HL53315, The Foundation Fighting Blindness, The Cleveland Clinic Foundation and Alcon Inc.
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