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E.C. LaHey, R. Diederen, N. Deutz, S. Dieudonne, A.T. A. Liem, A. Kijlstra, F. Hendrikse; Excess of glutamate in vitreous from eyes with retinal detachment. . Invest. Ophthalmol. Vis. Sci. 2004;45(13):2059.
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Purpose:Excess of glutamate, as may occur in association with hypoxia, is neurotoxic. The aim of our study was to examine whether rhegmatogenous retinal detachment is associated with increased levels of intraocular glutamate. Methods:Undiluted vitreous samples were obtained from 176 patients undergoing pars plana vitrectomy in our clinic between 1999 and 2003. Vitreous concentrations of glutamate were determined using high–pressure liquid chromatography (HPLC), and were compared to vitreous levels of GABA and 23 other amino acids. The study group consisted of vitreous fluid samples from eyes with retinal detachment (n=114). As controls we used vitreous from eyes with idiopathic macular hole (n=26), idiopathic epiretinal membrane (n=26), and vitreous from eyes with proliferative diabetic retinopathy (PDR) (n=10). Results: The level of glutamate in vitreous fluid from eyes with retinal detachment (16.6 ± 5.6 µM) was significantly higher compared to the control group (13.1 ± 5.2 µM) (P< .0001). The mean level of glutamate in vitreous fluid from patients with PDR (19.5 ± 6.5 µM) was even higher, and also significant compared to the control group (13.1 ± 5.2 µM) (P<0.0001). Conclusions: In the current study, rhegmatogenous retinal detachment was associated with a significant increase in vitreous glutamate. Whether this results from a release by degenerative retinal neurons or from an impaired uptake of glutamate due the retinal ischemia associated with retinal detachment needs further study.
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