Purchase this article with an account.
I. Kawaguchi, T. Higashide, S. Ohkubo, K. Sugiyama; In vivo monitoring of retinal nerve fiber layer by scanning laser ophthalmoscope in rat models with ganglion cell damage . Invest. Ophthalmol. Vis. Sci. 2004;45(13):2146.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose:Although a number of reports showed retinal ganglion cell death in rat retinal ischemia/reperfusion or optic nerve crush models, changes of retinal nerve fiber layer (RNFL) were not well documented. The purpose of this study is to examine the changes of RNFL in vivo with a scanning laser ophthalmoscope (SLO) in both models. Methods:Male Brown–Norway rats, 12 weeks of age and initially weighing 200–250g, were used. Following experiments were conducted under intraperitoneal anesthesia with 50 mg/kg of sodium pentobarbital. 1. Retinal ischemia/reperfusion model (n=4). The anterior chamber of the right eye was cannulated with a 30–gauge needle connected to a saline container. Retinal ischemia was induced by pressurizing the container to 140 mmHg, and was confirmed by indirect ophthalmoscopy. After 45 min of ischemia, the intraocular pressure was normalized. The left eye served as non–treated control. 2. Optic nerve crush model (n=7). The conjunctiva of the right eye was incised to expose the optic nerve by careful blunt dissection. The optic nerve was crushed for 30 seconds by a cross–action calibrated forceps (60gr pressure). Sham operation was performed in the left eye.3. Recording of RNFL was performed before and 1,2,4,8 weeks after retinal ischemia or optic nerve crush. The fundus was monitored by SLO with a fundus contact lens and argon laser, and the focus and the alignment were adjusted to obtain maximum reflex of RNFL. Results:All ischemia–induced eyes showed diffuse, severe loss of retinal nerve fiber bundles 1 week after injury. Any nerve fiber loss was not present in all eyes with optic nerve crush 1 week after injury, but diffuse loss was observed 1 week later and increased until 8weeks after injury. The control eyes showed no obvious changes in RNFL. Conclusions: SLO is useful to detect retinal nerve fiber loss of rat eye in vivo. Diffuse loss of retinal nerve fiber bundles occurs earlier in retinal ischemia/reperfusion model than in optic nerve crush model.
This PDF is available to Subscribers Only