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J.R. McColm, P. Geisen, M.E. Hartnett; Expression of VEGF and PEDF in a Rat Model of ROP . Invest. Ophthalmol. Vis. Sci. 2004;45(13):2494.
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Purpose: To determine the expression of VEGF and PEDF in a rat model of ROP. Methods: A well defined rat model of ROP was used that cycled oxygen between 50% and 10% every 24 hours for 14 days. Newborn litters were subjected to this oxygen regime for various time periods and then euthanized. Controls were room air raised. Retinas were dissected from the animals and flat mounted and stained with Griffonia lectin to visualize the vasculature, stored in RNAlater for RT–PCR or in M–Per for ELISA. Results: This model results in an avascular peripheral retina at p14 (mean area 30%) and if the animals are left in room air for 4 more days, typical neovascularization is seen at the vascular avascular interface. Results are given in the table (units for the RT–PCR are arbitrary units based on densitometry standardized to 18S RNA).
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