May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Supression of fibrotic responses by Mycophenolic Acid in Human Tenon Fibroblasts in Vitro
Author Affiliations & Notes
  • R. Amer
    Ophthalmology, Hadassah University Hospital, Jerusalem, Israel
  • I. Puxeddu
    Pharmacology, School of Pharmacy, Faculty of Medicine, The Hebrew Univeristy, Jerusalem, Israel
  • I. Anteby
    Ophthalmology, Hadassah University Hospital, Jerusalem, Israel
  • F. Levi–Schaffer
    Pharmacology, School of Pharmacy, Faculty of Medicine, The Hebrew Univeristy, Jerusalem, Israel
  • A. Solomon
    Ophthalmology, Hadassah University Hospital, Jerusalem, Israel
  • Footnotes
    Commercial Relationships  R. Amer, None; I. Puxeddu, None; I. Anteby, None; F. Levi–Schaffer, None; A. Solomon, None.
  • Footnotes
    Support  Israel Ministry of Health – Chief Scientist Grant
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 2949. doi:
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      R. Amer, I. Puxeddu, I. Anteby, F. Levi–Schaffer, A. Solomon; Supression of fibrotic responses by Mycophenolic Acid in Human Tenon Fibroblasts in Vitro . Invest. Ophthalmol. Vis. Sci. 2004;45(13):2949.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:To evaluate the effects of mycophenolic acid (MPA) on human tenon fibroblast proliferation, apoptosis and necrosis in vitro. Methods:Human tenon fibroblasts were obtained from tissue explants from young healthy patients during strabismus surgery. Proliferation of subconfluent fibroblasts was assessed by using the (³H) thymidine–incorporation assay. Fibroblast proliferation was assessed following TGF–ß1 stimulation at the optimal concentration of 10 ng/ml. MPA was added (10–3M to 10–8 M in DMEM with 2% fetal calf serum) to the cultures for 24 hours. Fibroblast apoptosis and necrosis induced by MPA was assessed by the addition of annexin–V and propidium iodide stains, and by evaluating positively stained cell populations with FACS. Results:MPA significantly inhibited TGF–ß1–induced fibroblast proliferation with optimal concentrations ranging from 10–3 to 10–6 M. The addition of MPA at 10–3 M suppressed TGF–ß1–induced fibroblast proliferation by 10% (p<0.001). MPA did not cause apoptosis nor did it induce significant necrosis of the fibroblast populations under study. Conclusions:Mycophenolic acid demonstrated a significant antiproliferative effect on human tenon fibroblasts in vitro. This effect is not the result of cytotoxic or apoptotic effect of the drug. This effect can be utilized to modify fibrotic responses of the ocular surface in various cicatrizing and allergic disorders, as well as in postoperative fibrosis in pterygium excision and in glaucoma filtering surgey.

Keywords: conjunctiva • drug toxicity/drug effects • proliferation 
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