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J. Pastor, M. de la Fuente, R. Sanabria, E. Rodríguez de la Rúa, J. García–Arumí, M. Garcia, M. Mendez, N. Fernandez, M. Saornil; Immunohistochemistry findings in intraretinal proliferative vitreoretinopathy (iPVR) . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3195.
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Purpose: We have recently published the existence of retinal shortening (intraretinal PVR), in cases of PVR, besides the presence of preretinal and/or subretinal membranes. We now seek to analyze the retina specimens obtained in those cases that required a retinectomy to reattach the retina. Methods: Samples from 13 patients with postoperative iPVR, developed within the 2 first months after primary surgery for retinal detachment (RD), have been analyzed for the presence of: Vimentin, Cytokeratins (CKs), Glial Fibrillary Acidic Protein (GFAP) and CD68 by immunohistochemistry. A normal posterior pole from the eye–bank and 2 globes removed after complicated RD surgery, with PVR, were used as controls Results: Positive staining for Vimentin and GFAP was shown in 13/13 samples in the retinal tissue; remainings of epiretinal membranes attached to the retina were present in 10/13 cases, all negative for GFAP; 4/13 retinal tissues showed pigmented cells positive for CKs; 3/10 epiretinal membranes were also positive for CKs; 11/13 retinal tissues, 6/10 epiretinal membranes and 2 vitreous remains showed CD68 positive cells. Conclusions: Retinal shortening due to iPVR is caused by a gliotic response, a mechanism different from that implicated in epiretinal membranes. The presence of macrophages in retinal tissues indicates a possible important role of these cells in the pathogenesis of iPVR. Nevertheless, further studies with larger samples are necessary
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