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L. Chen, R. Levinson, J. Vaudaux, K. Lampi, R. Goldhardt, G.N. Holland, L.K. Gordon; Serum Antibodies Against Beta B1 Crystallin in Uveitis: Correlation with Posterior Subcapsular and Cortical Cataracts . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3375.
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Purpose: Beta B1 crystallin (ßB1) was identified as a candidate autoantigen in uveitis using archival samples from patients with anterior uveitis. The cataract status in this population was not known. This study was designed to quantify seroreactivity against ßB1 in a well–characterized uveitis population in order to define a possible correlation between cataract and anti–ßB1 seroreactivity in these patients. Methods: Serum from 40 uveitis patients, in whom the degree of lens opacity was graded (LOCS III classification), was collected under human subjects approval. Recombinant ßB1 protein was used as an antigenic target in a slot blot test for antibody reactivity. An internal positive and negative serum control panel was used. Positive anti–ßB1 reactivity (anti–ßB1+) was defined as greater than the mean + 2SD of the negative controls. Statistical analysis was performed using Chi–Square and Student’s t test. Results: In the uveitis sera tested, 65% had significant antibody reactivity against ßB1. The mean age of the anti–ßB1+ and the negative anti–ßB1 reactivity (anti–ßB1–) groups was identical at 47.4 years. No correlation existed between anti–ßB1 reactivity and uveitis activity at the time of serum collection. Significant correlation was observed between higher grades of posterior subcapsular or cortical cataract and seroreactivity against ßB1 (p < 0.05 for both), however,there was no correlation with higher grade of nuclear sclerotic cataract. Conclusions: A majority of uveitis patients in this study demonstrated significant reactivity against ßB1. The degree of reactivity did not reflect the level of intraocular inflammation nor was it associated with age–related cataract. Seroreactivity against ßB1 was more likely in patients with a higher grade of cortical or posterior subcapsular cataract as judged by LOCS III. Further studies will define the relationship of this reactivity with cataract pathogenesis
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