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T. Nakamura, F. Ishikawa, K.–H. Sonoda, T. Hisatomi, H. Qiao, J. Yamada, T. Ishibashi, M. Harada, S. Kinoshita; Distribution and Characterization of Bone Marrow–Derived Cells in the Mouse Cornea . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3518.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Recent studies suggested that bone barrow–derived stem cells have a tremendous differentiative capacity. The purpose of this study was to investigate the distribution and characterization of bone marrow–derived cells in the normal mouse cornea. Methods: Bone marrow cells (BMC) and hematopoietic stem cells (HSC) (lin (–), Sca–1 (+)) were obtained from enhanced GFP transgenic mice. They were transplanted into wild type C57BL/6 mice that had been lethally X–rays irradiated. These BMC and HSC transplantation mice were maintained under special pathogen free conditions, successful BM transplantation was confirmed by the identification of GFP–positive cells in the blood two to four weeks after irradiation. At 6 months after transplantation, we killed the mice and then, the whole corneas were examined for histological and immunohistochemical study (CD11c, CD11b and Iab). Results: At two weeks after BMC transplantation, GFP positive cells gradually migrated into the cornea from the limbal area and finally, these cells were distributed in the whole cornea at 6 month after transplantation. Cross sections of the whole cornea revealed that 27.3% (BMC) and 24.0% (HSC) GFP positive cells / total cells were observed in the peripheral corneal stroma and 7.6% (BMC) and 8.0% (HSC) GFP positive cells / total cells were observed in the center of corneal stroma. Immunohistochemistry showed that GFP(+) CD11c(+) cells, GFP(+) CD11b(+) cells and GFP(+) Iab(+) cells were observed in the whole corneal area. Meanwhile, GFP(+) CD11c(–) cells, GFP(+) CD11b(–) cells and GFP(+) Iab(–)cells were also observed in it. Conclusions: The present study demonstrated that bone marrow–derived cells indeed distributed in the mouse cornea which is generally thought to be avascular tissue. From the immunohistochemical study, these cells were the bone marrow–derived antigen presenting cells such as dendritic cells and macrophages; otherwise some part of cells might be other cell lineage such as corneal keratocyte. Further immunohistochemical study is needed to clarify this point.
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