Purchase this article with an account.
S. Jung, S. Choi, E.–K. Kim, C.Y. Im, J. Park, J.V. Jester; TGF–beta 1 concentration is increased after UVB irradiation in hTERT immortalized human fibroblasts . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3783.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: The Avellino corneal dystrophy (ACD), one of the Big–H3 gene related dystrophy, begins at the interpapebral fissure where cornea is exposed to sun–light even after squint. As Big–H3 gene is activated by TGF–beta, the concentration of TGF–beta 1 was checked from the culture media after UVB irradiation of the keratocytes to find out the possible relation between ACD and UVB. Methods: Immotalized human fibroblasts (hTERT, extended–life human corneal fibroblasts) were cultured with DMEM containing 10% FBS and irradiated with 0, 5, 10, 15 and 30 mJ/cm2 UVB. Culture supernatants were collected at 0, 4, 8, 24, and 48 hours after UVB irradiation and TGF–beta 1 production was assayed by Enzyme–Linked Immunosorbant Assay (ELISA). Analysis of co–variance (ANCOVA) was performed after log transformation of TGF–beta 1 values. Results: Production of TGF–beta 1 protein in culture supernatants assayed by ELISA was increased at 10, 15 and 30 mJ/cm2 UVB irradiation (p<0.05). Conclusions: UVB irradiation increases the level of TGF–beta 1 in the corneal stroma. Increased TFG–beta 1 would activate Big–H3 gene to produce more abnormal keratoepithelin and may be a factor in the progression of ACD. TGF–beta 1 concentration (pg/ml) after UVB irradiation
This PDF is available to Subscribers Only