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T. Rodriguez–Ares, M.J. López–Valladares, R. Touriño, M. Silva, E. Becerra, B. Vieites; Characteristics of Lyophilized Human Amniotic Membrane . Invest. Ophthalmol. Vis. Sci. 2004;45(13):3918.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To describe histological changes observed in human amniotic membrane preserved by freeze–drying, as part of a study to assess freeze–drying as a method to preserve amniotic membrane. Methods: Human placentas were obtained aster elective cesarean deliveries from donors seronegative. After being washed and separated from corion, amniotic membrane was laid onto a cellulose nitrate filter and cut in pieces. This pieces went under lyophilization following a program previously validated (freeze–dryer VIRTIS Genesis). Small samples of freeze–dried human amniotic membrane were examined with light microscope after hematoxylin and eosine dye. To estimate cellular viability amniotic membranes were immersed in propidium iodide and calcein AM. Results: Histopathological exam showed no significant morphologic alteration after freeze–drying in human amniotic membrane, keeping epithelial cells and basement membrane with their characteristics. The observation with the fluorescence microscope demonstrated that there was not cellular viability. Conclusions: Freeze–drying keeps histological structure of human amniotic membrane and could be a good alternative as a preservation method for human amniotic membrane until surgery, though more studies are necessary to determine what happen with the concentration of cytokines in which lay most of the observed clinical effects of human amniotic membrane.
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