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E. Hoyama; Acellular dermal graft study: An ultrastructural evaluation of human and porcine derived tissues in a rat model . Invest. Ophthalmol. Vis. Sci. 2004;45(13):4709.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: The purpose of this study was to evaluate the host response of a human (T1) and a porcine (T2) derived acellular dermal tissue (ADT) implanted in the subcutaneous tissue of a rat model. Methods: Two subcutaneous pockets were surgically created along the dorsal midline of 25 rats (5 rats/group). The T1 was placed superiorly and the T2, inferiorly. The animals were sacrificed at 07 (M1), 15 (M2), 30 (M3), 60 (M4) and 180 (M5) postoperative days (PO) and the ADTs and surrounding soft tissues were assessed for ultrastructural evaluation by transmission electron microscopy. Results: : The ultrastructural findings were similar in both materials. Normal collagen and elastic fibers bundles were observed during all experimental moments, as well as macrophages presenting cytoplasmic enlargements digesting cellular portions after M2. From M3 until M5, vacuolar structures filled with an amorphous, electron–transparent substance, were present inside and outside the fibroblasts. Conclusions: Both T1 and T2 showed similar pattern of ultrastructural response when implanted in the subcutaneous tissue of rats. The T2 appears as a good alternative to be used as a biomaterial.
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