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K.–C. Yoon, K.–K. Kim, K.–Y. Ahn, D.–W. Lim, M.–S. Seo, Y.–G. Park; Inhibition of Corneal Neovascularization by Brain–specific Angiogenesis Inhibitor 1 Gene Delivery . Invest. Ophthalmol. Vis. Sci. 2004;45(13):4815.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To investigate the antiangiogenic effects of the subconjunctival injection of brain–specific angiogenesis inhibitor 1 (BAI1) gene in a rabbit model of corneal neovascularization. Methods: One week after epithelial debridement by heptanol, the rabbits were treated with the subconjunctival injection of BAI1 gene or vector. The injection was done two or three times at one week interval. Rabbit not received any injection after debridement served as pharmacological control. To assess the gene expression the the transfected rabbit cornea after the subconjunctival injection, injection of the gene carried by pEGFP vector or pEGFP–BAI1 gene was performed 1 week after debridement. Analysis of corneal neovascularization was performed by biomicroscopy and immnuohistochemical examination of VEGF staining. Results: Fluorescent microscopy of the cornea showed green fluorescence detected distinctly in corneal stroma of both groups. One and two weeks after two injections of BAI1 or vector, BAI1–treated eyes had 43.4% and 41.0% less neovascularized corneal area than vector–treated eyes (P=0.02, P=0.01), and 51.5% and 51.1% less neovascularized area than no treated eye (P=0.04, P=0.05), respectively. One and two weeks after three injections of BAI1 or vector, BAI1–treated eyes had 52.1% and 49.4% less neovascularized corneal area than vector–treated eyes (P=0.01, P=0.02), and 48.2% and 45.4% less neovascularized area than no treated eye (P=0.03, P<0.01), respectively. In histological examination, the BAI1–treated group showed significantly less sectioned neovascularized area and less number of vessels than other groups. Conclusions: BAI1 gene delivery effectively reduces corneal neovascularization and can be useful as angiogenesis inhibitor in the cornea.
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