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K. Izumi, D. Kurosaka, Y. Mashima, T. Taya, N. Osada, Y. Oguchi, Y. Tanaka, T. Iwata; cDNA Microarray Analysis of Gene Expression in Cultured Human Corneal Fibroblasts following TGF–ß2 Stimulation . Invest. Ophthalmol. Vis. Sci. 2004;45(13):4875.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:To identify changes in gene expression of human corneal fibroblasts after exposure to TGF–ß2. Methods:RNA was isolated from cultured human corneal fibroblasts after exposure to TGF–ß2 and hybridized to a human cDNA microarray containing 12,646 genes. Gene–specific semi–quantitative reverse transcription–polymerase chain reaction (RT–PCR) was used to confirm the gene expression pattern measured by the cDNA expression microarray (Agilent Technologies). Results:After exposure to TGF–ß2, the expression of 1,264 genes was significantly up–regulated and the expression of 762 genes was down–regulated in human corneal fibroblasts. Semi–quantitative RT–PCR analysis confirmed the TGF–ß2–mediated changes in expression patterns of these genes. Conclusions:We have analyzed the effects of gene expressions by TGF–ß2 treatment on human corneal fibroblasts using cDNA microarray. Transcription of genes coding cytoskeleton, ECM, and adhesion were significantly affected by TGF–ß2.
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