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M.I. Pinilla, N. Cuenca, Y. Sauvé, S. Wang, B. Lu, R.D. Lund; Effects of photoreceptor loss on relative changes in rod and cone pathways in the retina of P23H transgenic rats. . Invest. Ophthalmol. Vis. Sci. 2004;45(13):5071.
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Purpose: To examine how rod photoreceptor degeneration in P23H rats impacts on rod and cone relay pathways in the inner retina. Methods:P23H Line 1 homozygote albino rats of 21, 40, 150, and 270 days and control albino Sprague–Dawley (SD) rats of 21, 38, and 150 days were studied anatomically and by ERG recording. Results:The loss of rods generated changes in their postsynaptic neurons. There was early loss of dendrites in rod bipolars, with associated reduction of mGluR6 and rod–associated bassoon staining, but cone pathways seemed relatively unaffected. With time there was secondary sprouting related to horizontal cells; attendant with these clusters, mGluR6 and bassoon staining were found. ERG results at 21 days indicate a reduction in the amplitudes of a–waves and isolated rod and cone b–waves (using a double flash paradigm) and decrease in the amplitude of oscillatory potentials (OPs). By 38 days a–wave thresholds were elevated and OPs had vanished. Increase in b–wave thresholds was first seen at 148 days. Conclusions:During the course of photoreceptor loss in the P23H rat, there are both degenerative and reactive changes occurring in the inner retina, which are reflected at the ERG level.
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