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E.–J. Lim, E.–J. Lee, I.–B. Kim, W.–S. Kang, S.–J. Oh, M.–H. Chun; The calretinin immunoreactive ganglion cells postsynaptic to the ON–cholinergic amacrine cell in the guinea pig retina . Invest. Ophthalmol. Vis. Sci. 2004;45(13):5373.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To characterize calretinin immunoreactive cells in ganglion cell layer (GCL) of the guinea pig retina. Methods: Vibratome sections were double–labeled using antisera against calretinin (1:1000) and the following antibodies: anti–ChAT (1:200; Chemicon), and anti–alpha1 or beta2/3 GABAA receptor antibodies (1:100; Chemicon). Results: Calretinin immunoreactivity is present in displaced amacrine cells and ganglion cells with processes that ramify in stratum 4 of the inner plexiform layer (IPL). The same stratum also occupied by the dendrites of ON–cholinergic amacrine cells. Double–labeling experiments demonstrated that dendrites and cell bodies of displaced amacrine cells colocalized with ON–cholinergic amacrine cells and dendrites of calretinin immunoreactive ganglion cells directly overlapped with dendrites of ON–cholinergic amacrine cells which are presynaptic to the DS ganglion cells and ON α ganglion cells. The synaptic connectivity of calretinin immunoreactive ganglion cells was identified in the IPL by electron microscopy. The calretinin immunoreactive ganglion cell processes received synaptic input from ON–cholinergic amacrine cell processes in stratum 4 of the IPL. We have also found that ß2/3 subunit of GABAA receptor bands co–faciculates the dendrites of calretinin immunoreactive displaced amacrine cell and ganglion cell that are juxtapose to the a α1 subunit of GABAA receptor. Conclusions: These results indicate that ON–cholinergic amacrine cells modulate calretinin labeled ganglion cell via GABAA receptor, ß2/3, in the guinea pig retina.
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