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U.A. Schraermeyer, S. Arnhold, I. Semkova, S. Kochanek, H. Janicki, J. Kozlowski, K. Addicks, U. Bartz–Schmidt; Subretinally transplanted bone marrow stromal cells develop into retinal pigment epithelial–like cells. . Invest. Ophthalmol. Vis. Sci. 2004;45(13):5389.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:In this study we investigated the potential of subretinally transplanted bone marrow stromal cells (MSCs) to develop into retinal pigment epithelial (RPE) cells. Methods:An HC–Ad vector was constructed to express the enhanced green fluorescence protein (EGFP) from the human CMV promoter. This vector (HC–AdFK7) was used to transduce rat MSCs in cell culture before subretinal injection into Wistar rats. In vivo expression of EGFP was monitored by stereo fluorescence microscopy. Transplanted rats were sacrificed 2 months following cell transplantation, and retinal flatmount preparations and electron microscopy were performed. Immuno cytochemistry for cytokeratin and tight junction protein ZO1 was also done. Results:MSCs from rats integrated into the host RPE cell layer indicated by their hexagonal shape. The transplanted cells expressed the epithelial cell marker cytokeratin in the same manner as the host RPE cells and formed tight junctions with the host RPE cells. In addition they also phagocytosed remnants of rod outer segments as observed by electron microscopy. Conclusions:These results raise the possibility that stem cell–enriched MSCs have the ability to replace diseased RPE cells and to deliver therapeutic proteins into the subretinal space.
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