May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Transcorneal Electrical Stimulation Enhances the Survival of Axotmized Retinal Ganglion Cells In Vivo
Author Affiliations & Notes
  • T. Morimoto
    Physiology and Biosignaling, Osaka Univ Grad Sch Med, Suita, Japan
  • T. Miyoshi
    Physiology and Biosignaling, Osaka Univ Grad Sch Med, Suita, Japan
  • T. Fujikado
    Opthalmology, Osaka Univ Grad Sch Med, Suita, Japan
  • Y. Tano
    Opthalmology, Osaka Univ Grad Sch Med, Suita, Japan
  • Y. Fukuda
    Opthalmology, Osaka Univ Grad Sch Med, Suita, Japan
  • Footnotes
    Commercial Relationships  T. Morimoto, None; T. Miyoshi, None; T. Fujikado, None; Y. Tano, None; Y. Fukuda, None.
  • Footnotes
    Support  Health Sciences Research Grants in Japan
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 109. doi:
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    • Get Citation

      T. Morimoto, T. Miyoshi, T. Fujikado, Y. Tano, Y. Fukuda; Transcorneal Electrical Stimulation Enhances the Survival of Axotmized Retinal Ganglion Cells In Vivo . Invest. Ophthalmol. Vis. Sci. 2003;44(13):109.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Previously we demonstrated the neuroprotective effect of electrical stimulation to the transected optic nerve (ON) on axotomized retinal ganglion cells (RGCs) in adult rats (Morimoto et al, 2002). In this study, we introduced transcorneal electrical stimulation (TES) as a less invasive method and evaluated the neuroprotective effect of TES on axotomized RGCs. Methods: Adult male Wistar rats were used. Seven days after injection of Fluorogold (FG), a fluorescent tracer for retrograde labeling, into the superior colliculi, the left ON was transected ~3 mm from the posterior eye pole. TES was applied through concentric bipolar electrodes on a contact lens for 1 hour immediately after ON cut. The electrical stimuli were biphasic current pulses (1ms/phase, 100µA) of 20 Hz. One week after ON transection, mean RGC densities were calculated by the number of FG-labeled neurons counted in 12 areas covering the whole retina. Results: In normal retinas, the mean RGC density was 2368 ± 182 cells/mm2(mean ± SD). In ON transection group and sham stimulation group, the mean RGC densities significantly decreased to 53% and 51% of the density of normal retinas, respectively. On the other hand, TES increased the densities of surviving RGCs to 82% of normal value. The neuroprotctive effect of TES was comparable to that of TES to the transected ON. Conclusions: TES enhanced the survival of axotomized RGCs in adult rats. TES can be a new neuroprotective therapy for damaged RGCs.

Keywords: neuroprotection • ganglion cells • apoptosis/cell death 
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