May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
2-deoxy-D-glucose Protects Retinal Ganglion Cells against N-methyl-D-aspartate Mediated Excitotoxicity
Author Affiliations & Notes
  • Y. Lan
    Ophthalmology, Jules Stein Eye Institute, Los Angeles, CA, United States
  • J.M. Kwong
    Ophthalmology, Jules Stein Eye Institute, Los Angeles, CA, United States
  • Y. Ishii
    Ophthalmology, Jules Stein Eye Institute, Los Angeles, CA, United States
  • J. Caprioli
    Ophthalmology, Jules Stein Eye Institute, Los Angeles, CA, United States
  • Footnotes
    Commercial Relationships  Y. Lan, None; J.M.K. Kwong, None; Y. Ishii, None; J. Caprioli, None.
  • Footnotes
    Support  RPB
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 133. doi:
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      Y. Lan, J.M. Kwong, Y. Ishii, J. Caprioli; 2-deoxy-D-glucose Protects Retinal Ganglion Cells against N-methyl-D-aspartate Mediated Excitotoxicity . Invest. Ophthalmol. Vis. Sci. 2003;44(13):133.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To investigate the neuroprotective effect of 2-deoxy-D-glucose (2DG) on the retinal ganglion cells (RGCs) against excitotoxicity induced by N-methyl-D-aspartate (NMDA). Methods: Male albino Sprague-Dawley rats, 40-45 days old, were given daily intraperitoneal injections of 2DG (200 mg/kg) or saline. After 7 days of 2DG pretreatment, 2µl of 4mM NMDA was injected intravitreally. At 7 days after NMDA injection, animals were euthanized and the enucleated eyeballs were bisected. The temporal retinas were dissected for flat preparation and cresyl violet-stained neurons in the RGC layer were counted. Immunohistochemistry with antibody against Thy-1 was preformed on the nasal eyecups and the number of Thy-1 positive cells in the RGC layer per retinal section was evaluated. Results: Intravitreal injection of NMDA induced a loss of 44% cresyl violet-stained neurons and 38% Thy-1 positive cells in the RGC layer. The pretreatment of 2DG significantly ameliorated the loss of neurons (15% loss) and Thy-1 positive cells (8% loss) in the RGC layer. There was no significant difference in cell counting between vehicle and 2DG-treated controls. No significant weight loss after 2DG pretreatment was observed. Conclusions: 2DG, a non-metabolizable glucose analog that mimics caloric restriction, protects retinal ganglion cells against NMDA-mediated excitotoxicity. Supported by RPB Commercial Relationships Policy: N

Keywords: neuroprotection • excitatory neurotransmitters • retina: proximal(bipolar, amacrine, and gangli 
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